Development of a human iPSC-derived placental barrier-on-chip model

•A 3D perfused placental barrier with high integrity differentiated from hiPSC•On-chip differentiation improved the spatial organization and directionality•Detection of endocrine, fusogenic markers transporters•3D microfluidic format boosted mechanosensing cell surface receptor signaling Although recently developed placenta-on-chip systems opened promising perspectives for modeling, they still lack physiologically relevant trophoblasts are poorly amenable to high-throughput studies. We aimed implement human-induced pluripotent stem cells (hiPSC)-derived into a multi-well device develop scalable model. When cultured in micro-channel against collagen-based matrix, hiPSC-derived self-arranged structure showing invasive behavior, endocrine activities, structural integrity, expressing transporters. RNA-seq analysis revealed that environment expression genes related early development, mainly involved signaling. 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